What You Need to Know About Taking a Pregnancy Test at Night

Is it 10 o’clock at night and it’s just hit you that you’re five days late on your period?

Have you and your hubby been trying to conceive and you’re wondering if it’s okay to take a pregnancy test as soon as you get off work because the suspense is just killing you?

If you’re feeling any symptoms of early pregnancy, then it may be time to take a home pregnancy test. But when is the best time to take one? Will it be accurate if you take it at night?

Keep reading to find out.

How Do Home Pregnancy Tests Work?

Home pregnancy tests work by detecting the human chorionic gonadotropin (hCG) hormone in your urine. This hormone rapidly produces in the first few days of pregnancy after the embryo implants into your uterine wall. HCG helps form the placenta and supports the development of your growing baby.

Most home pregnancy tests have you either collect your urine in a cup and dip the stick into your urine or place the stick in your urine midstream. Some even have you collect urine and insert it into a well on the stick with an eye dropper.

You then have to lay the test on a flat surface and wait for a designated period (usually between 3-5 minutes). After this time, you will look for a change in color, a line, or a symbol that will indicate if you are indeed pregnant or not (source).

Related Reading: What You Should Know About Expired Pregnancy Tests

When Is the Best Time to Take a Home Pregnancy Test?

One of an essential rule of a home pregnancy tests it when to take it. Most home pregnancy tests claim to detect a pregnancy as early as 3-4 days before your missed period. But for the most accurate results, healthcare providers recommend waiting until one week after your missed period (source).

You may even experience some pregnancy symptoms before even taking a test, such as:

If you’re experiencing any symptoms of early pregnancy, it is certainly okay to take a pregnancy test sooner. Just know that you may get a false negative from taking it too early, meaning that you get a negative result, but are in fact pregnant.

This will happen because you may not have enough hCG in your urine to be detectable. If you get a negative result but think you’re pregnant, wait 5-7 days and retake the test.

Can I Take a Home Pregnancy Test at Night?

All home pregnancy tests are designed to detect hCG, but there are certain times of the day in which it’s more detectable.

No matter which tests you buy, your first-morning urine will always give you the most accurate results, as your urine is more concentrated, allowing hCG levels to be higher and more easy to detect.

However, there is no one stopping you from taking a home pregnancy test at night. If you really can’t wait until morning, then go ahead and take one.

The only issue with taking one at night is that it might be inaccurate.

If the test comes back positive when taking it at night, then you are most likely pregnant, as it is pretty rare to get a false-positive result (but it does happen).

False positives are usually the result of an ectopic pregnancy, early miscarriage, fertility drugs, or user error (reading the test too soon/late or misinterpreting the evaporation line as a positive line).

If your test comes back negative, just try again in the morning. You may get a different result with your first-morning urine, as there may have been too little of the hCG hormone in your urine at night to trigger a positive result.

What About a Blood Test?

Blood tests also work by detecting the hcG hormone but can detect a pregnancy much sooner (about 7-12 days from conception) than a urine test.

A blood test should give you the same result no matter what time of day, but they are expensive, have to be done at a doctor’s office, and take longer to get results back (source).

Related Reading: Faint Line Pregnancy Test: What Does It Mean?

Get Testing

  • If you think you might be pregnant, but too anxious to wait until morning to take a pregnancy test, then go ahead and take one. Just know that taking a pregnancy test at night could give you inaccurate answers.
  • If you take a pregnancy test at night and get a positive result, then you are most likely pregnant. If you get a negative result, you might not be pregnant, but you may have also just not had high enough levels of hCG in your urine to be detectable.
  • If you get a negative result, go ahead and take another test in the morning. You may get a different result with your first-morning urine. Good luck, and I hope you get the results you’re wanting!

Have you taken a home pregnancy test at night? Did you get accurate results? Share your experiences with us in the comments and be sure to share this post all of your friends that are trying to conceive.

16 Reader Comments

  1. Racial Dcruzz

    ALWAYS buy two tests. Unless you are a relentlessly optimistic person or a happily naive person, one test will not be enough.

  2. Juna

    Hi, I took a pregnancy test last night with a positive result. I then took another test this morning and it was negative. Why would this happen? Am I pregnant?

    • Jenny Silverstone

      Pregnancy tests can be tricky. If you took two different types of tests, they might detect different levels of HCG. This means one might show positive before the other does. It’s also possible that the test you took in the evening showed an evaporation line instead of a positive. Did you read the results within the allotted time frame? I recommend waiting a few days and testing again to make sure.

  3. Rachel

    So I am 10 weeks pregnant and had mixed positives and negatives then it hit me! All the negatives were using fmu, so I decided to test my theory I took 2 tests in the morning both negative then took 2 test at 7pm and both positive as soon as urine passed over the test! It turns out my HCG levels were too high to be detected in the morning, but by 7 pm I had drunk 4 bottles of water and 3 cans of coke, so the more diluted my urine was, the stronger the result!
    My GP told me about the hook effect which is what has happened to me. I may be further along in pregnancy or carrying multiples. Will find out at my scan!

    • Jenny Silverstone

      Thanks for sharing Rachel! That’s really interesting. Congratulations and good luck with your pregnancy 🙂

  4. Shannan

    Had a positive at night about 7 pm took 3 tests (with different pees) and all had a very faint line and then in the morning and all the next day( I have a problem lol). I got negative so not sure what’s going on. Will have to wait and see in a couple of days and do another one. The wait is killing me though, lol.

  5. It’s crazy how well a woman knows her body. I had a feeling I was pregnant, and my partner insisted that there was no way like he always did. He assumed his sperm wasn’t sufficient. I took a test, and it came back negative, but I knew it had to be wrong.

    Soon after, implantation spotting happened, and I knew for sure. He still denied it but went and got two more pregnancy tests to put me at ease. I took one that night after work, and it was negative. I took the second in the morning, and sure enough, it was positive. I’m almost six weeks pregnant. I also made sure to tell him “I told you!”

    • Jenny Silverstone

      Thank you for sharing your story, and congratulations on your pregnancy!

  6. Abhinetri

    I took a test at night, but I got a false positive, even though I think that I am pregnant. What should I do?

    • Jenny Silverstone

      What makes you think that the positive test was a false positive? Did you take another test in the morning with negative results? If you think are pregnant I would wait a few days and test again. Good luck!

  7. I have gotten 2 negative results but I have all the symptoms that come with pregnancy. I had only a light period each of the last two months as well. Could I be pregnant?

    • Jenny Silverstone

      Hi Dee,
      I recommend waiting a few days and testing again. Sometimes it can take a little while for the HCG to build up. Have you considered going to your doctor to ask for a blood test?

  8. Karina

    So last night around 10 pm I took a test and it came out positive. It had one dark line and one very light line. Today I took another test when I woke up and it came out negative. I do not have any symptoms, but I haven’t gotten my period yet either. Am I pregnant?

    • Jenny Silverstone

      Hi Karina,

      It’s possible that the line you saw last night was an evaporation line, but since your period is late it is also possible that you are pregnant. I recommend waiting a few days and testing again if your period has not started. Were both tests the from the same brand?


  9. Melanie

    With my first pregnancy, we had just decided we were ready to try for a baby. One evening, I poured myself a glass of wine and was put off by the first sip! The smell, the taste! Weird! I started to do the math in my head and realized I was about five days late which was unusual for me. I happened to have a pregnancy test on hand so I took it and it turned out positive. I think it was about 7 pm. I took another one the next morning, the same result!

    • Jenny Silverstone

      Thank you for sharing your story, Melanie! Have you tried testing at night with any of your other pregnancies?

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